25 Point Website Usability Checklist
This 25-point usability checklist is really an excellent solution to understand the basic principles of a perfectly optimized and managed site

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ACCESSIBILITY
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Site load-time is reasonable
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Adequate text-to-background contrast
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Font size/spacing is easy to read
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Flash & add-ons are used sparingly
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Images have appropriate ALT tags
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Site has custom not-found/404 pace
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IDENTITY
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Company Logo Is Prominently Placed
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Navigation label are clear concise
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Number of buttons/links Reasonable
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Company logo linked to home-page
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Links are consistent & Easy to identify
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Site Search Is Easy to Access
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CONTENT
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Major headings are Clear & descriptive
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Critical content above the fold
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Styles & colors are consistent
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Emphasis (bolo, etc.) used sparingly
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ADS & pop-ups are unobtrusive
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Main copy is concise & Explanatory
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URLs are meaningful & User-friendly
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Html page titles are explanatory